Composite

Part:BBa_K1702002:Design

Designed by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson   Group: iGEM15_CSU_Fort_Collins   (2015-09-17)


E. coli fadD and fadL under control of lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2939
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression of fadD and fadL is inducible with lactose or IPTG.


Source

fadL and fadD were extracted using genomic PCR from DH5alpha E. coli. The lac promoter was sourced from the iGEM distribution kit.

References