Composite
Part:BBa_K1702002:Design
Designed by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson Group: iGEM15_CSU_Fort_Collins (2015-09-17)
E. coli fadD and fadL under control of lac promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2939
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The expression of fadD and fadL is inducible with lactose or IPTG.
Source
fadL and fadD were extracted using genomic PCR from DH5alpha E. coli. The lac promoter was sourced from the iGEM distribution kit.